Journal: The journal of pain : official journal of the American Pain Society

Article Title: Enrichment of genomic pathways based on differential DNA methylation associated with chronic postsurgical pain and anxiety in children – a prospective, pilot study

doi: 10.1016/j.jpain.2018.12.008

Figure Lengend Snippet: Overlap between CpG sites associated with chronic postsurgical pain (CPSP) and childhood anxiety sensitivity index (CASI) with functional genomics datasets in cells derived from brain tissue

Article Snippet: Roadmap Epigenomics (Histone narrow) , H1 Derived Neuronal Progenitor Cells , H3K4me2 , 0.283 , 0.0002.

Techniques: Functional Assay, Derivative Assay

Journal: Toxins

Article Title: Structure Elucidation and Biological Evaluation of Maitotoxin-3, a Homologue of Gambierone, from Gambierdiscus belizeanus

doi: 10.3390/toxins11020079

Figure Lengend Snippet: Comparison of the effects of CTX3C, gambierone and MTX3 on cell viability in human cortical neurons. None of the toxins affected CTX0E16 cell viability at low concentrations. In this case, the maximum toxin concentration evaluated was 20 nM, and toxicity was evaluated after exposure of the cells to the different compound concentrations for 5 days in vitro. Cell viability was evaluated by the MTT assay. Results are expressed as mean ± sem of 4 independent experiments, each performed in triplicate.

Article Snippet: The neuronal stem cell line CTX0E16 was immortalized from the cerebral cortex of a 12 weeks of gestation fetus, by the ectopic expression of the c-mycER TAM transgene and kindly provided by a material transfer agreement with ReNeuron Limited (Guildford, Surrey, UK).

Techniques: Comparison, Concentration Assay, In Vitro, MTT Assay

Journal: Toxins

Article Title: Structure Elucidation and Biological Evaluation of Maitotoxin-3, a Homologue of Gambierone, from Gambierdiscus belizeanus

doi: 10.3390/toxins11020079

Figure Lengend Snippet: Long term exposure (15 days in culture) of human differentiated cortical neurons to either gambierone or MTX3, at 20 nM, affected the protein level of glutamate receptor subunits. ( A ) Both compounds decreased the protein level of the AMPA receptor subunits. Western blot bands showing the expression of GluR2,3,4 subunit proteins are shown on the left panel and the corresponding quantifications of the bands are shown on the right. ( B ) The same treatments were performed to evaluate the effect of the compounds on the expression of NMDA receptor subunits NR2A/B. Representative western blot bands showing NR2A/B levels in differentiated CTX0E16 neurons are shown on the left panel and band intensities quantifications are shown on the right. Fifteen days exposure of human cortical neurons to 20 nM gambierone or 20 nM MTX3 increased NR2A/B levels, and effect opposite to that previously described for CTX3C in mice cortical neurons. The number of independent determinations is shown in parenthesis. ** p < 0.01 versus control.

Article Snippet: The neuronal stem cell line CTX0E16 was immortalized from the cerebral cortex of a 12 weeks of gestation fetus, by the ectopic expression of the c-mycER TAM transgene and kindly provided by a material transfer agreement with ReNeuron Limited (Guildford, Surrey, UK).

Techniques: Western Blot, Expressing, Control